Journal: Oncotarget

Article Title: Clinical and biological significance of miR-23b and miR-193a in human hepatocellular carcinoma

doi: 10.18632/oncotarget.14332

Figure Lengend Snippet: SKHep1C3 cells were treated with DMSO (vehicle) and 10 μM 5-aza-dC for 5 days (lanes 1 and 2) or treated with 10 μM 5-aza-dC in combination to 100 nM miR-193a transfection (lanes 5 and 8) and relatives controls (lanes 3, 4, 6 and 7) at 24h and 48h from transfection. Western blot was performed to detect MET and GAPDH expression A . on cell extracts and uPA expression B . and corresponding enzymatic activity C . on conditioned medium. The histograms represent the mean IOD (Integrated Optical Density) values in percentage while bars are SEM. *p<0.05 using unpaired t-test analysis.

Article Snippet: The following primary antibodies were used: rabbit anti-human MET (1:1000 in 0.3% BSA; Santa Cruz Biotecnology), mouse monoclonal antibodies anti-GAPDH (1:500 in 1% BSA; Merck Millipore) and rabbit anti-human uPA (1:1000 in 1% BSA; Technoclone).

Techniques: Transfection, Western Blot, Expressing, Activity Assay